Head of the Transgenic Core Facility, MPI of Molecular Cell Biology and Genetics, Dresden/Germany
Title: Generating a mouse-rat chimeric brain by murine GFP-ES
Biography: Mr.Ronald Naumann
The Transgenic Core Facility at the MPI-CBG provides a centralized resource and state-of-the-art technology in production of Knock-Out mice by injection or aggregation of embryonic stem cells into mouse embryos, and transgenic mice by injection of DNA into the pronuclear of mouse oocytes. The Service provides in addition CRISPR methods into cells and mouse zygotes.
In the actual application it shown a pilot project to established a chimeric brain by injection of mouse ES-cells into a rat embryo.
All neurons generated during the development of the mammalian CNS derive from neuroepithelial (NE) cells. NE cells undergo three principal kinds of cell division, symmetric, proliferative divisions (two NE cells), asymmetric divisions (one NE cell, one neuron) and symmetric, differentiating divisions (two neurons). The rat neurogenesis is very close to human brain development.
Because the neurogenesis works different between rat and mice – we generate a chimeric model which shows both specific neuro development in only one tissue.